By Helena Jane Maier, Erica Bickerton, Paul Britton
This quantity goals to explain numerous concepts that displays the wide variety of study at the moment played within the box of coronavirology, and starts with an summary of present understandings of coronavirus replication and pathogenesis to introduce experts and non-specialists to the sphere. the remainder of the ebook is split into numerous sections of chapters starting with those who describe identity, analysis, and examine of the evolution of coronaviruses. the following couple of chapters speak about the training of cells and organ cultures necessary in propagating coronaviruses and titration recommendations, in addition to innovations for examining virus features that require purification of the viruses. the subsequent chapters describe primary opposite genetics thoughts for coronaviruses, and methods detailing identity of mobile receptors, binding profiles of viral attachment proteins, and virus-cell fusion. the ultimate chapters conceal a huge spectrum of options to spot virus-host protein-protein interactions, ascertain the practical function of those proteins in virus replication, learn host mobilephone responses via genome-wide or pathway-specific techniques, and visualize virus replication complexes. Written within the hugely winning Methods in Molecular Biology series layout, the chapters comprise the type of specified description and implementation recommendation that's the most important for purchasing optimum ends up in the laboratory.
Authoritative and useful, Coronaviruses: tools and Protocols appeals to a large choice of scientists since it highlights strategies which are at the moment utilized in the coronovirology box, whereas additionally discussing practices acceptable to different virology fields.
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Additional resources for Coronaviruses: Methods and Protocols
Williams GD, Chang RY, Brian DA (1999) A phylogenetically conserved hairpin-type 3′ untranslated region pseudoknot functions in coronavirus RNA replication. J Virol 73:8349–8355 46. Hsue B, Masters PS (1997) A bulged stemloop structure in the 3′ untranslated region of the genome of the coronavirus mouse hepatitis virus is essential for replication. J Virol 71:7567–7578 47. Hsue B, Hartshorne T, Masters PS (2000) Characterization of an essential RNA secondary structure in the 3′ untranslated region of the murine coronavirus genome.
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Immunchromatographic Test for Serological Diagnosis of FCoV 39 References 1. Hohdatsu T, Okada S, Koyama H (1991) Characterization of monoclonal antibodies against feline infectious peritonitis virus type II and antigenic relationship between feline, porcine, and canine coronaviruses. Arch Virol 117:85–95 2. Motokawa K, Hohdatsu T, Aizawa C et al (1995) Molecular cloning and sequence determination of the peplomer protein gene of feline infectious peritonitis virus type I. Arch Virol 140:469–480 3.
Coronaviruses: Methods and Protocols by Helena Jane Maier, Erica Bickerton, Paul Britton
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