By Souleymane Bado, Brian P. Forster, Abdelbagi Ghanim, Joanna Jankowicz-Cieslak, Berthold Günter, Liu Luxiang
This ebook bargains powerful, inexpensive and basic protocols for the pre-field choice of salt-tolerant mutants in cereal vegetation. It provides basic tools for measuring soil salinity, together with soil sampling and the research of water-soluble salts, and describes an in depth, yet basic, screening try out for salt tolerance in rice, wheat and barley seedlings, which makes use of hydroponics. The protocols are devised to be used by means of plant breeders and will be simply accommodated into breeding perform.
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This booklet deals potent, inexpensive and effortless protocols for the pre-field collection of salt-tolerant mutants in cereal plants. It provides uncomplicated tools for measuring soil salinity, together with soil sampling and the research of water-soluble salts, and describes a close, yet uncomplicated, screening try for salt tolerance in rice, wheat and barley seedlings, which makes use of hydroponics.
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Extra resources for Protocols for Pre-Field Screening of Mutants for Salt Tolerance in Rice, Wheat and Barley
1007/978-3-319-26590-2_5 33 34 5 Protocol for Screening for Salt Tolerance in Barley and Wheat synchronous germination. Solutions of sodium hypochlorite can be easily made from commercial bleach (about 5 % NaClO). 8 % NaClO for 20 min and then washing three times with water. Seed (up to 50) may be placed onto wetted filter paper in Petri dishes (4 ml water per 9 cm Petri dish, water should not cover the seed). The Petri dishes are then placed in the dark at 4 C (fridge) for 48 h; this low temperature facilitates uniform germination.
Solutions can be changed by lifting off the platforms and placing them temporarily onto empty tanks and pouring the hydroponic solutions back into a drum where the bulked solution can be pH adjusted for the whole experiment in one step. Once adjusted, the solution is redistributed into the test tanks and the seedling platform returned. These operations also act to aerate the hydroponic solution. Alternatively, the pH can be adjusted on an individual tank basis, and more working solution may be added to make up the volume in each tank.
After 5–6 days, seedlings of uniform development are removed and placed into hydroponics. Alternatively, pretreated seed in Petri dishes may be transferred to room temperatures (17–25 C) in the light for another 48 h, replenishing water when necessary (when the Petri dish is tilted, there should be an excess of about 1 ml water). After 4 days in the light, germinated seeds should have the first leaf emerging from the coleoptile and 3–8 roots (Fig. 1b). These seedlings are removed individually and placed inside a sponge collar and inserted into the hydroponic system (Fig.
Protocols for Pre-Field Screening of Mutants for Salt Tolerance in Rice, Wheat and Barley by Souleymane Bado, Brian P. Forster, Abdelbagi Ghanim, Joanna Jankowicz-Cieslak, Berthold Günter, Liu Luxiang
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